Enzymes

EC Number

1.1.1.49

CAS Number

9001-40-5

Catalyzed reaction(s)

D-glucose 6-phosphate + NADP⁺ = 6-phospho-D-glucono-1,5-lactone + NADPH + H⁺

Synonyms

G6PDH, 6-phosphoglucose dehydrogenase, D-glucose 6-phosphate dehydrogenase, Entner-Doudoroff enzyme, G6PD, GPD, NADP-dependent glucose 6-phosphate dehydrogenase, D-glucose-6-phosphate:NADP⁺ 1-oxidoreductase

Origin

Bacterial

Molecular weight

45.8 kDa

Other characteristics

Thermostable up to 80°C

Production

Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.

Formulation

Liquid, buffer with 50 % (v/v) glycerin

Storage

-20°C

Example reaction conditions

N/A

Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.