Enzymes
Hydrolysis
Ribonucleoside hydrolase NH01
Art.-No.: E-NH-0001
Escherichia coli rihA, mesophilic
290,00 €
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The ribonucleoside hydrolase NH01 corresponds to the Escherichia coli rihA gene. Ribonucleoside hydrolases catalyze the irreversible hydrolysis of nucleosides to ribose and nucleobase. NH01 is stricktly specific for ribonucleosides and accepts the pyrimidine nucleosides cytidine and uridine with equal efficiency.
EC Number
3.2.2.8
CAS Number
37288-60-1
Catalyzed reaction(s)
pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Synonyms
Ec-RihA, RNH, Ec-YbeK, ribosylpyrimidine nucleosidase, pyrimidine nucleoside hydrolase, N-ribohydrolase, N-ribosylpyrimidine nucleosidase, pyrimidine-nucleoside ribohydrolase
Origin
Escherichia coli
Molecular weight
35.2 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Ribonucleoside hydrolase NH02
Art.-No.: E-NH-0002
Escherichia coli rihB, mesophilic
290,00 €
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The ribonucleoside hydrolase NH02 corresponds to the Escherichia coli rihB gene. Ribonucleoside hydrolases catalyze the irreversible hydrolysis of nucleosides to ribose and nucleobase. NH02 is stricktly specific for ribonucleosides and accepts the pyrimidine nucleosides cytidine and uridine with clear preference for cytidine over uridine. Purine ribonucleosides are also accepted, but conversion is much slower.
EC Number
3.2.2.8
CAS Number
37288-60-1
Catalyzed reaction(s)
pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Synonyms
Ec-RihB, RNH, Ec-YeiK, ribosylpyrimidine nucleosidase, pyrimidine nucleoside hydrolase, N-ribohydrolase, N-ribosylpyrimidine nucleosidase, pyrimidine-nucleoside ribohydrolase
Origin
Escherichia coli
Molecular weight
35.1 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Ribonucleoside hydrolase NH03
Art.-No.: E-NH-0003
Escherichia coli rihC, mesophilic
290,00 €
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The ribonucleoside hydrolase NH03 corresponds to the Escherichia coli rihC gene. Ribonucleoside hydrolases catalyze the hydrolysis of nucleosides to ribose and nucleobase. NH03 hydrolyzes both purine and pyrimidine ribonucleosides with a broad substrate specificity. Activity decreases in the order uridine > xanthosine > inosine > adenosine > cytidine > guanosine. The enzyme is strictly specific for ribonucleosides.
EC Number
3.2.2.1/3.2.2.8
CAS Number
9025-44-9/37288-60-1
Catalyzed reaction(s)
(1) purine nucleoside + H2O = D-ribose + purine base
(2) pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Synonyms
Ec-RihC, RNH, Ec-YaaF, non-specific ribonucleoside hydrolase, purine-nucleoside ribohydrolase
Origin
Escherichia coli
Molecular weight
34.0 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
Uridine hydrolase NH04
Art.-No.: E-NH-0004
Eukaryotic origin, mesophilic
290,00 €
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The uridine hydrolase NH04 is a mesophilic enzyme of eukaryotic origin. The enzyme catalyzes the hydrolysis of pyrimidine ribonucleosides to ribose and nucleobase with a clear preference for uridine.
EC Number
3.2.2.3
CAS Number
9025-47-2
Catalyzed reaction(s)
uridine + H2O = D-ribose + uracil
Synonyms
UH, uridine nucleosidase, uridine ribohydrolase
Origin
Eukaryotic
Molecular weight
39.4 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
MTA/SAH nucleosidase NH06
Art.-No.: E-NH-0006
Escherichia coli mtnN, mesophilic
290,00 €
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The 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase NH06 corresponds to the Escherichia coli mtnN gene. The enzyme catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. It also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine and accepts 5'-isobutylthioadenosine, 5'-n-butylthioadenosine, S-adenosyl-D-homocysteine, decarboxylated adenosylhomocysteine, deaminated adenosylhomocysteine and S-2-aza-adenosylhomocysteine as well as purine ribonucleosides in vitro.
EC Number
3.2.2.9
CAS Number
9055-10-1
Catalyzed reaction(s)
(1) S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
(2) 5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
(3) S-methyl-5'-thioadenosine + H2O = 5-(methylsulfanyl)-D-ribose + adenine
(4) purine nucleoside + H2O = D-ribose + purine base
Synonyms
Ec-MtnN, MTAN, SAHN, Ec-YadA, 5'-methyladenosine nucleosidase, S-methyl-5'-thioadenosine/S-adenosyl-L-homocysteine nucleosidase, S-adenosylhomocysteine hydrolase, S-adenosylhomocysteine nucleosidase, AdoHcy/MTA nucleosidase, S-adenosyl-L-homocysteine homocysteinylribohydrolase
Origin
Escherichia coli
Molecular weight
25.8 kDa
Other characteristics
Mesophilic
Production
Recombinant from E. coli, purified by His-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Formulation
Liquid, buffered, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
